Academic Background

August 2012 – December 2016

PhD Candidate, Chemistry, University of Michigan, Ann Arbor, MI, USA

Advisers: Professors A. Ramamoorthy and Mi Hee Lim

Aug. 2008 – May 2012 Biochemistry, Valparaiso University, Valparaiso, IN

Graduated Summa Cum Laude

Research project

I am applying biochemical, biophysical, and NMR techniques to probe the folding and misfolding of Alzheimer's related amyloid beta (Ab) to answer the following questions:

1) How do rationally designed small molecules alter the folding and structure of Ab species?

2) How does Ab misfold on membrane bilayers and how does this correlate to cell toxicity?

3) What role does membrane composition play in the formation of Ab aggregates?

Publications

15) Jones, M. R.; Mathieu, E.; Dyrager, C.; Faissner, S.; Vaillancourt, X.; Korshavn, K. J.; Lim, M. H.; Ramamoorthy, A.; Yong, V. W.; Tsutsui, S.; Stys, P. K.; Storr, T. Multi-target-Directed Phenol-Triazole Ligands as Therapeutic Agents for Alzheimer's Disease. Chem. Sci. 2017. Accepted for Publication.

14) Lee, H. J.; Korshavn, K. J.; Nam. Y.; Kang, J.; Paul, T. J.; Kerr, R. A.; Youn, I. S.; Ozbil, M.; Kim, K. S.; Ruotolo, B. R.; Prabhakar, R.; Ramamoorthy, A.; Lim, M. H. Structural and Mechanistic Insights into Development of Chemical Tools to Control Individual and inter-related Pathological Features in Alzheimer's Disease. Chem. Eur. J. 2017, 23, 2706-2715. LINK

13) Kang, J.; Lee, S. J. C.; Nam, J. S.; Lee, H. J.; Kang, M.-G.; Korshavn, K. J.; Kim, H.-T.; Cho, J.; Ramamoorthy, A.; Rhee, H.-W.; Kwon, T.-H.; Lee, M. H. An Iridium(III) Complex as a Photoactivatable Tool for Oxidation of Amyloidogenic Peptides with Subsequent Modulation of Peptide Aggregation. Chem. Eur. J. 2017, 23, 1645-1653. LINK

12) Korshavn, K. J.; Satriano, C.; Lin, Y.; Zhang, R.; Dulchavsky, M.; Bhunia, A.; Ivanova, M. I.; Lee, Y. H.; La Rosa, C.; Lim, M. H.; Ramamoorthy, A. Reduced Lipid Bilayer Thickness Regulates the Aggregation and Cytotoxicity of Amyloid-b. J. Biol. Chem. 2017, 292, 4638-4650. LINK

11) Bera, S.*; Korshavn, K. J.*; Kar, R. K.; Lim, M. H.; Ramamoorthy, A.; Bhunia, A. Biophysical Insights into the Membrane Interaction of the core amyloid-forming Ab40 Fragment K16-K28 and its Role in the Pathogenesis of Alzheimer's Disease. Phys. Chem. Chem. Phys. 2016, 22, 16890-16901 (* Denotes co-first authorship). LINK

10) Jones, M. R.; Dyrager, C.; Hoarau, M.; Korshavn, K. J.; Lim, M. H.; Ramamoorthy, A.; Storr, T. Multifunctional Quinoline-triazole Derivatives as Potential Modulators of Amyloid-b Peptide Aggregation. J. Inorg. Biochem. 2016, 158, 131-138. LINK

9) Derrick, J. S.; Kerr, R. A.; Korshavn, K. J.; McLane, M. J.; Kang, J.; Nam, E.; Ramamoorthy, A.; Ruotolo, B. T.; Lim, M. H. Importance of the Dimethylamino Functionality on a Multifunctional Framework for Regulating Metals, Amyloid-b, and Oxidative Stress in Alzheimer's Disease. Inorg. Chem. 2016, 55, 5000-5013. LINK

8) Lee, H. J.; Kerr, R. A.; Korshavn, K. J.; Lee, J.; Kang, J.; Ramamoorthy, A.; Ruotolo, B. T.; Lim, M. H. Effects of Hydroxyl Group Variations on a Flavonoid Backbone Toward Modulation of Metal-Free and Metal-Induced Amyloid-b Aggregation. Inorg. Chem. Front. 2016, 3, 381-392. LINK

7) Korshavn, K. J.; Bhunia, A.; Lim, M. H.; Ramamoorthy, A. Amyloid-b Adopts a Conserved, Partially Folded Structure upon Binding to Zwitterionic Lipid Bilayers Prior to Amyloid Formation. Chem. Comm. 2016, 52, 882-885. (Work featured on cover art) LINK

6) Korshavn, K. J.; Jang, M.; Kwak, Y. J.; Kochi, A.; Vertuani, S.; Manfredini, S.; Ramamoorthy, A.; Lim, M. H. Reactivity of Metal-Free and Metal-Associated Amyloid-b with Glycosylated Polyphenols and Their Esterified Derivatives. Sci. Rep. 2015 DOI: 10.1038/srep17842. LINK

5) Derrick, J. S.; Kerr, R.; Nam, Y.; Oh, S. B.; Lee, H. J.; Earnest, K. G.; Suh, N.; Peck, K. L.; Ozbil, M.; Korshavn, K. J.; Ramamoorthy, A.; Prabhakar, R.; Merino, E. J.; Shearer. J.; Ruotolo, B.; Lim, M. H.: Covalent Link of a Redox-Active, Compact Molecule for the Crosslinking of Amyloidogenic Peptides into Nontoxic, Off-pathway Aggregates. J. Am. Chem. Soc. 2015 137, 14785-14797. LINK

4) Lee, H. J.^*; Korshavn, K. J.^*; Kochi, A.; Derrick, J. S.; Lim, M. H. Cholesterol and Metal Ions in Alzheimer’s Disease. Chem. Soc. Rev. 2014, 6672-6682 ( ^* denotes co-first authorship). LINK

3) Beck, M.W.; Pithadia, A.S.; DeToma, A.S.; Korshavn, K. J.; Lim, M. H. Chapter 10: "Ligand Design to Target and Modulate Metal-Protein Interactions in Neurodegenerative Diseases" in "Ligand Design in Medicinal Inorganic Chemistry" (John Wiley & Sons, LTD., Chinchester, West Sussex PO 19 8SQ), 2014. LINK

2) Savelieff, M. G. ; Liu, Y. ; Senthamarai, R. R. P. ; Korshavn, K. J. ; Lee, H. J. ; Ramamoorthy, A. ; Lim, M. H. ; A Small Molecule that Displays Marked Reactivity Toward Copper- versus Zinc- Amyloid-b Implicated in Alzheimer’s disease. Chem. Commun. 2014, 50, 5301-5303. LINK

1) Yu, G. ; Bayer, A. R. ; Galloway, M. M. ; Korshavn, K. J. ; Fry, C. G. ; Keutsch, F. N. Glyoxal in Aqueous Ammonium Sulfate Solutions: Products, Kinetics and Hydration Effects. Environ. Sci. Tech. 2011, 45, 6336-6342. LINK

Conferences

6) Korshavn, K. J.; Bhunia, A.; Lim, M. H.; Ramamoorthy, A. ; The Effects of Zwitterionic Bilayer Thickness on the Aggregation Kinetics and Structure of Ab₄₀, Poster award winner, 2015 Karle Symposium, University of Michigan, Ann Arbor, MI, August 7, 2015.

5) Korshavn, K. J.; Bhunia, A.; Lim, M. H.; Ramamoorthy, A. ; The Effects of Zwitterionic Bilayer Thickness on the Aggregation Kinetics and Structure of Ab₄₀, Poster , FASEB Conference: Molecular Mechanisms and Physiological Consequences of Protein Aggregation, West Palm Beach, FL, June 2015.

4) Korshavn, K. J.; Bhunia, A.; Lim, M. H.; Ramamoorthy, A. ; The Effects of Zwitterionic Bilayer Thickness on the Aggregation Kinetics and Structure of Ab₄₀, Selected Speaker, Gordon Research Conference: Proteins, Holderness, NH, June 2015.

3) Korshavn, K. J.; Kochi, A.; Manfredini, S.; Ramamoorthy, A.; Lim, M. H. ; Insights into the Activity of a set of Glycosylated Polyphenols and Their Synthetic Derivatives Against Metal-Ab Species, Poster, 2014 Vaughan Symposium, University of Michigan, Ann Arbor, MI, July 30, 2014.

2) Savelieff, M. G. ; Liu, Y. ; Senthamarai, R. R. P. ; Korshavn, K. J. ; Lee, H. J. ; Ramamoorthy, A. ; Lim, M. H. ; A Small Molecule that Displays Marked Reactivity Toward Copper- versus Zinc- Amyloid-b Implicated in Alzheimer’s disease, Poster, 37th Annual Steenbock Symposium, University of Wisconsin: Madison, Madison, WI, June 6, 2014.

1) Savelieff, M. G. ; Liu, Y. ; Senthamarai, R. R. P. ; Korshavn, K. J. ; Lee, H. J. ; Ramamoorthy, A. ; Lim, M. H. ; A Small Molecule that Displays Marked Reactivity Toward Copper- versus Zinc- Amyloid-b Implicated in Alzheimer’s disease, Poster, Amyloid and Human Disease Symposium, Ann Arbor, MI, May 8, 2014.