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Stéphanie Le Clair

leclairs@umich.edu


Department of Chemistry
930 N. University Ave.
Ann Arbor, MI, 48109

(734) 763-9318

Education

Chemistry Ph.D. Candidate

M.S. in Analytical Chemistry, University of Michigan, 2009

B.S. in Chemistry, minor in Mathematics, Saint Mary's College of California, 2006

Research

Investigating the structure and dynamics of the cytochrome b5-cytochrome P450 complex in different membrane environments.

Honors and Awards

  • NSF Graduate Research Fellowship (2009-2012)
  • NIH Molecular Biophysics Training Grant Fellowship (2008-2009)
  • Regents' Fellowship (2008)
  • Graduated Saint Mary's College of California Summa Cum Laude (2006)
  • Arthur S. Campbell Award - Oustanding scholarship (highest GPA) in the School of Science (2006)
  • Joseph P. McKenna Award - Outstanding scholarship (highest GPA) as a chemistry major (2006)
  • ACS Science and Engineering Outstanding Performance Award in Organic Chemistry (2004)
  • CRC Press Freshman Chemistry Achievement Award (2003)
  • Honors at Entrance Scholarship (2002-2006)

Poster Presentations

  • "Understanding the Dynamic Nature of Redox Protein Complexes using Solution Nuclear Magnetic Resonance." The Vaughan Symposium, Ann Arbor, MI, July 28, 2011.
  • “NMR Structural Studies of the Interactions between Mutants of Mammalian Microsomal Cytochrome b5 and Cytochrome P450.” Experimental Nuclear Magnetic Resonance Conference, Asilomar Conference Grounds, Pacific Grove, CA, April 10-15 2011.
  • “NMR Structures Provide Insights into the Pathology of Islet Amyloid Polypeptide and SEVI Peptide.” Experimental Nuclear Magnetic Resonance Conference, Asilomar Conference Grounds, Pacific Grove, CA, March 29 - April 3, 2009.
  • “Structural Characterization of the Membrane Toxicity of Human Islet Amyloid Polypeptide.” Perspectives in Chemistry Research at The University of Michigan, Ann Arbor, Michigan, August 1, 2008. Received a Vaughan Symposium award for outstanding poster.
  • “Structural Characterization of the Membrane Toxicity of Human Islet Amyloid Polypeptide.” Central Regional Meeting of the American Chemical Society, Columbus, OH, June 2008.

Publications

  • Nguyen K; Le Clair, Stéphanie V.; Ye, Shuji; and Chen, Zhan. “Orientation Determination of Protein Helical Secondary Structure Using Linear and Nonlinear Vibrational Spectroscopy.” Journal of Physical Chemistry B. 2009, 113, 12169-12180.
  • Nguyen K; Le Clair, Stéphanie V.; Ye, Shuji; and Chen, Zhan. “Molecular Interaction between Magainin 2 and Model Membranes in Situ.” Journal of Physical Chemistry B. 2009, 113, 12358-12363.
  • Ye, Shuji; Nguyen K; Le Clair, Stephanie V.; and Chen, Zhan. “In Situ Molecular Level Studies on Membrane Related Peptides and Proteins in Real Time Using Sum Frequency Generation Vibrational Spectroscopy.” Journal of Structural Biology. 2009, 168, 61-77.
  • Le Clair, Stéphanie V.; Nguyen K; and Chen, Zhan. "Sum Frequency Generation Studies on Bioadhesion: Elucidating the Molecular Structure of Proteins at Interfaces.” Journal of Adhesion. 2009, 85, 484-511.
  • Esmonde-White, Karen A.; Le Clair, Stephanie V.; Roessler, Blake J.; Morris, Michael D. “Effect of Conformation and Drop Properties on Surface-Enhanced Raman Spectroscopy of Dried Biopolymer Drops.” Appl. Spectrosc. 2008, 62, 503-511.

Research Experience

University of Michigan                                                                                                Ann Arbor, MI

Graduate Research Assistant, January 2008-December 2009

Department of Chemistry and Biophysics, Ayyalusamy Ramamoorthy and Zhan Chen, advisors

Research focused on amyloidogenic peptides/proteins’ interactions with model bilayers using SFG and FTIR-ATR

  • Studied the orientation of IAPP in model bilayers using SFG and FTIR-ATR to understand its mode of interaction with the bilayer


Graduate Research Assistant, September 2007-December 2007

Departments of Chemistry and Biophysics, Hashim Al-Hashimi, advisor

The project’s objective was to do a method validation of ICM for docking ligands to RNA molecules

  • Picked suitable RNA-ligand crystal structures from the PDB database
  • Determined whether ICM could highly score and rank known binders to their respective RNA molecule
  • Studied whether ICM could properly predict the conformation of ligands within the RNA binding pocket, and the amount of searching required to do this
  • Analyzed ICM’s ability to predict binding scores that correlated to Kd values for each RNA-ligand complex

 

Summer Intern, June 2007-August 2007

Department of Chemistry, Michael Morris, advisor

Research focused on finding a new optical way to study dried droplets of synovial fluid

  • Began designing a Raman system in which a ring-disc illumination laser beam could be obtained through a microscope, with an adjustable diameter size, to study dried droplets of synovial fluid

 

Argonne National Laboratory                                                                                                                       Argonne, IL

SULI Intern (D.O.E.),January 2007 – April 2007

Energy Systems, Paula Moon, mentor

Used electrodialysis to find a new effective method to de-salt produced water so that the water may be re-used

  • Performed experiments using electrodialysis to remove ions from Powder River Basin produced water
  • Tested different electrodialysis membrane types, current densities and stock concentrations to find an optimum for each variable
  • Carried out different post-demineralization treatments to determine which was most effective at de-salting the water, after use of the electrodialysis system
  • Wrote a report entitled “Integrated Electrodialysis Membrane Process for Beneficial Use of Coalbed Methane Produced Water”

 

S.U.L.I. Intern (D.O.E.),September 2006 – April 2007

Energy Systems, Joe Pomykala, mentor

Supported shredder residue separation technologies for solid-solid and solid-liquid mixtures

  • Performed experiments using novel surface modification techniques, such as froth flotation, to separate dissimilar plastics from one another
  • Characterized the physical chemistry and composition of aqueous solutions using analytical instrumentation such as tensiometers, hydrometers and pH meters
  • Identified different types of plastics recovered from shredder residue using FTIR-ATR
  • Conducted experiments to remove PCBs from recovered plastics using extraction solutions and thermal desorption
  • Wrote a report entitled “Separating and Recovering Materials from Shredder Residue”

 

Roche Palo Alto LLC                                                                                                                                 Palo Alto, CA

Summer Intern, June 2006 – August 2006                                

Drug Metabolism and Pharmacokinetics, Ludmila Alexandrova and Bill Fitch, mentors

Participated in the metabolism studies of a back-up compound for the treatment of cognitive disorders

  • Carried out rat liver microsome incubations with and without GSH to study reactive metabolite formation
  • Isolated and purified three hydroxy metabolites and five GSH adducts from rat liver microsome incubation mixtures, using SPE and HPLC-UV
  • Proposed and confirmed structures of these metabolites via LC-MS and NMR
  • Proposed metabolic pathways of hydroxy metabolites and GSH conjugation with the drug molecule
  • Identified several possible reactive sites of the drug compound
  • Presented results in a PowerPoint presentation

 

Summer Intern, June 2005 – August 2005

Analytical Chemistry Department, Yan Liu and Joseph Pease, mentors

Drug Metabolism and Pharmacokinetics, Ludmila Alexandrova and Bill Fitch, mentors   

Participated in the metabolism studies of a lead compound for the treatment of cognitive disorders

  • Performed routine HNMR and 13CNMR analyses on drug compounds
  • Isolated and purified three metabolites from human and monkey urine utilizing SPE and HPLC-UV
  • Elucidated the structures of those metabolites by LC-MS and NMR
  • Presented results in a PowerPoint presentation

 

Saint Mary’s College of California                                                                                      Moraga, CA

Research Assistant, previously Honors Special Study, September 2005 – August 2006                                                                   

Department of Chemistry, Michelle Shulman, advisor

Research focused on developing a novel method to analyze atmospheric samples using SPME/HPLC-UV

  • Developed HPLC-UV conditions to study atmospherically relevant water-soluble organics
  • Explored the uses of different SPME fibers and adsorption/desorption conditions to better concentrate the polar organics and enhance their detection
  • Summarized the SPME/HPLC-UV method developed and results in a report


Honors Special Study , January 2006

Department of Chemistry, Michelle Shulman, advisor

  • Designed and constructed realistic crime scenes for a forensic science course using a range of evidence types
  • Supervised students with on-the-scene and laboratory portions of evidence analysis

 

Work Experience

University of Michigan                                                                                                  Ann Arbor, MI

Graduate Student Instructor, Chemical Principles, May 2008 – June 2008

  • Designed, prepared and gave hour-long lectures on physical chemistry topics (to approximately 30 students)
  • Held office hours and review sessions

 Graduate Student Instructor, Intro to Chemical Analysis Lab, September 2007 – December 2007

  • Mentored juniors and seniors in a laboratory environment
 

Saint Mary’s College of California                                                                                      Moraga, CA

Tutor; Chemistry, Mathematics, French, and Anatomy, September 2004 – April 2006

  • Provided group and individual academic help, and led study sessions

Teacher’s Assistant; Human Anatomy, September 2005 – December 2005

  • Provided assistance in identification and close examination of cadavers and human models

Teacher’s Assistant; General Chemistry Lab I, II, February 2004 – May 2005                                                                           

  • Instructed the importance of following procedures, keeping detailed notebooks and stressed critical thinking
  • Guided students with calculations, data interpretation and use of Excel  

Library Computer Systems Student Assistant, September 2002 – May 2005  

  • Managed faculty profiles and updated college art slides, using PHP, HTML and Photoshop
  • Converted vinyl records to CDs in order to update the library’s selections
  • Mapped out computers and electrical outlets using Photoshop and MapEdit to centralize systems
  • Upgraded computer systems (ghosting, manipulating IP addresses, and changing settings)

Leadership Positions Held

  • Advisory Board Member of the Dean of Students (DOS) at the University of Michigan, Fall 2011 - Spring 2012
  • Peer Educator, through the Sexual Assault Prevention and Awareness Center, Ann Arbor, MI, Fall 2011 - Spring 2012
  • Advisory Board Member of the Office of Student Conflict Resolution (OSCR) at the University of Michigan, Winter 2011 - Spring 2012
  • Networking, Publicity and Activism volunteer, through the Sexual Assault Prevention and Awarenes Center, Ann Arbor, MI, Fall 2010

Leadership and Professional Development

  • 16-hour Division of Student Affairs Facilitation Training, Ann Arbor, MI, Fall 2012

  • 35-hour volunteer training at the Sexual Assault Prevention and Awareness Center, Ann Arbor, MI, Winter 2012
  • 40-hour Social Justice Mediation Training, led by Leah Wing and Deepika Marya (through the Social Justice Mediation Institute), offered by the Office of Student Conflict Resolution, Spring 2011
  • Intercultural Leadership Seminar, February 28 - March 5, 2011, Boyne City, MI

The Intercultural Leadership Seminar is an interactive 6-day program intended to provide participants with a deeper understanding of how to work more effectively in intercultural settings. The seminar design deliberately addresses attitudes, awareness, knowledge and skills as central aspects to developing intercultural competence. Because ILS attracts those who are concerned with cross-cultural issues, this seminar allows participants to have the unique opportunity to engage in intensive dialog with others from diverse backgrounds who share the commitment to increasing their own intercultural competence. The Intercultural Leadership Seminar is sponsored by the University of Michigan Alumni Association, the College of Engineering, and the International Center.

  • 40-hour volunteer training at the Sexual Assault Prevention and Awareness Center, Ann Arbor, MI, Fall 2010
  • LGBTQ Ally Training through the Spectrum Center, Ann Arbor, MI, Fall 2010

Memberships

  • American Chemical Society (ACS)
  • American Association for the Advancement of Science (AAAS)
  • Association for Women in Science (AWIS)
  • Phi Kappa Phi
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