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Thirupathi Ravula

travula@umich.edu 
Biophysics
930 N. University Ave.
Ann Arbor, MI 48109

(734) 763-9318



Academic Background:

I received my PhD from Indian Institute of Science (IISc), Bangalore, India. Currently working as post-doc in Prof. Ramamoorthy lab. My research interests includes nanomaterials, material chemistry, bionanomaterials, molecular self-assembly and computational chemistry.



Research:


Development of new polymer nanodisc : Nanodiscs are disc-shaped lipid bilayers surrounded by an amphiphilic protein and are inspired from high-density lipoproteins. Nanodiscs provide a near native like lipid membrane environment and enables the study of membrane proteins in its active formRecent advances in nanodiscs technology have allowed nanodiscs to be formed by scaffold proteins, amphiphilic peptide or polymer. The hydrolyzed form of styrene maleic anhydride copolymer was the first polymer shown to form nanodiscs (styrene maleic acid lipoparticles called as SMALPs), are versatile and promising tools in biophysical and biomedical research that can offer a detergent-free solubilization of membrane proteins maintaining their native lipid environment.  SMALPs have several disadvantages which limits their applications. In this lab we are developing new polymers that can form nanodisc for various applications.

Total internal reflection fluorescence (TRIF) microscopy showing the solubilization of  DMPC multilamellar vesicles (MLVs) containing DMPE-rhodamine B as a function of time after the addition of the SMA-EA polymer For details see :Bioinspired, Size-Tunable Self-Assembly of Polymer–Lipid Bilayer Nanodiscs



Solubilization of DMPC multilamellar vesicles ‎‎(MLVs)‎‎ with SMA-EA polymer








Publications:









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