Thirupathi Ravula, Ph.D.

travula@umich.edu

Education

2014 - Nanotechnology, Ph.D., Indian Institute of Science (IISc), Bengaloru, India

2008 - Chemistry, M.Sc., Pondicherry University, Pondicherry, India

Research Project

Development of new polymer nanodisc: Nanodiscs are disc-shaped lipid bilayers surrounded by an amphiphilic protein and are inspired from high-density lipoproteins. Nanodiscs provide a near native-like lipid membrane environment and enables the study of membrane proteins in their active form. Recent advances in nanodiscs technology have allowed nanodiscs to be formed by scaffold proteins, amphiphilic peptide or polymers. The hydrolyzed form of styrene-maleic anhydride copolymer was the first polymer shown to form nanodiscs (styrene-maleic acid lipoparticles called as SMALPs), are versatile and promising tools in biophysical and biomedical research that can offer a detergent-free solubilization of membrane proteins maintaining their native lipid environment. SMALPs have several disadvantages which limits their applications. In this lab we are developing new polymers that can form nanodisc for various applications.

Selected Publications

(18) 2019 - Hardin N., Kocman V., Di Mauro G., Ravula T., Ramamoorthy A., Metal-Chelated Polymer Nanodiscs for NMR Studies, Angewandte Chemie International Edition.

(17) 2019 - Hardin N., Ravula T., Di Mauro G., Ramamoorthy A., Hydrophobic Functionalization of Polyacrylic Acid as a Versatile Platform for the Development of Polymer Lipid Nanodisks, Small, 15, 1804813.

(16) 2019 - Hardin N., Ravula T., Di Mauro G., Ramamoorthy A., Hydrophobic Functionalization of Polyacrylic Acid as a Versatile Platform for the Development of Polymer Lipid Nanodisks, Small, 15, 1804813.

(15) 2019 - Ravula T., Ramamoorthy A., Magnetic-alignment of polymer macro-nanodiscs enable residual dipolar couplings based high-resolution structural studies by NMR, bioRxiv, 671982

(14) Barnaba C., Sahoo B.R., Ravula T., Medina-Meza, I.G., Im SC., Cytochrome-P450-Induced Ordering of Microsomal Membranes Modulates Affinity for Drugs, Angewandte Chemie International Edition, 57, 13, 3391-3395

(13) 2018 - Sumangala N., Mahajan M., Ravula T., Ramamoorthy A., Cytochrome-P450's Spin State Influences its Binding Affinity to its Redox Partner, Biophysical Journal 114, 3, 425a

(12) 2019 - Ravula T., Hardin N.Z., Ramamoorthy A., Polymer Nanodiscs: Advantages and Limitations, Chemistry and Physics of Lipids 2019, 45-49.

(11) 2018 - Ravula T., Hardin N., Di Mauro G., Ramamoorthy A., Styrene maleic acid derivates to enhance the applications of bio-inspired polymer based lipid-nanodiscs, European Polymer Journal, 108, 597-602.

(10) 2018 - Ravula T., Hardin N.Z., Bai J., Im S.C., Waskell L., Ramamoorthy A., Effects of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs, Chemical Communications 54 (69), 9615-9618.

(9) 2018 - Ravula T., Ishikuro D., Kodera N., Ando T., Anantharamaiah G.M., Ramamoorthy A., Real-Time Monitoring of Lipid Exchange via Fusion of Peptide Based Lipid-Nanodiscs, Chemistry of Materials 30, 10, 3204-3207.

(8) 2018 - Barnaba C., Sahoo B.R., Ravula T., Medina-Meza I.G., Im SC., Anantharamaiah G.M., Waskell L., Ramamoorthy A., Cytochrome-P450-Induced Ordering of Microsomal Membranes Modulates Affinity for Drugs, Angewandte Chemie International Edition 57, 13, 3391-3395

(7) 2018 - Sumangala N., Mahajan M., Ravula T., Ramamoorthy A., Cytochrome-P450's Spin State Influences its Binding Affinity to its Redox Partner, Biophysical Journal 114, 3, 425a

(6) 2018 - Ravula T., Hardin N.Z., Ramadugu S.K., Cox S.J., Ramamoorthy A., Formation of pH-Resistant Monodispersed Polymer-Lipid Nanodiscs, Angewandte Chemie International Edition 57, 5, 1342-1345.

(5) 2018 - Barnaba C., Ravula T., Medina-Meza I.G., Im SC. Anantharamaiah G.M., Waskell L., Ramamoorthy A., Lipid-exchange in nanodiscs discloses membrane boundaries of cytochrome-P450 reductase, Chemical Communications 54, 49, 6336-6339.

(4) 2017 - Yasuhara K., Arakida J., Ravula T., Ramadugu S.K., Sahoo B., Kikuchi J. Ramamoorthy A., Spontaneous lipid nanodisc formation by amphiphilic polymethacrylate copolymers, Journal of the American Chemical Society, 139, 51, 18657-18663.

(3) 2017 - Ravula T., Hardin N.Z., Ramadugu S.K., Ramamoorthy A., pH tunable and divalent metal ion tolerant polymer lipid nanodiscs, Langmuir 33, 40, 10655-10662.

(2) 2017 - Ravula T., Ramadugu S., Di Mauro G., Ramamoorthy A., Bioinspired Size-Tunable Self-Assembly of Polymer-Lipid Bilayer Nanodiscs, Angewandte Chemie International Edition 56, 38, 11466-11470.

(1) 2017 - Ravula T., Barnaba C., Mahajan M., Anantharamaiah G.M., Im SC., Waskell L., Ramamoorthy A., Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5, Chemical Communications 53, 95, 12798-12801.

Oral Presentations and Posters

N/A